DNA
PEBP-GFP

Part:BBa_K5291051:Design

Designed by: Yuning Wu   Group: iGEM24_BNUZH-China   (2024-10-01)


PEBP-GFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 895
    Illegal PstI site found at 905
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 895
    Illegal PstI site found at 905
    Illegal NotI site found at 109
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 895
    Illegal BamHI site found at 858
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 895
    Illegal PstI site found at 905
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 895
    Illegal PstI site found at 905
    Illegal NgoMIV site found at 1213
    Illegal NgoMIV site found at 1582
    Illegal AgeI site found at 256
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The expression way of this protein needs to be optimized.


Source

The sigal peptide and atuotranspoter in this part is from Pseudomonas. aeruginosa. And the PE binding peptide is predicted by modeling method.

References

Bergman, M. T., et al. (2023). "In Silico Design and Analysis of Plastic-Binding Peptides." The Journal of Physical Chemistry B 127(39): 8370-8381.